Effects of Phenyllactic Acid on Fermentation Parameters, Nitrogen Fractions and Bacterial Community of High-Moisture Stylo Silage

To investigate the effect of phenyllactic acid (PLA) on dynamic changes high-moisture stylo silage, fresh was ensiled with addition PLA at levels 0, 1% and 2% using lab-level silage bags, where samples were collected days 3, 7, 14 30 ensiling fermentation to analyze parameters, nitrogen distribution bacterial community. The results showed that led increase (p < 0.01) in dry matter content, lactic concentration Flieg’s score as well decrease loss, pH value coliform bacteria population, butyric only detected control group. It also resulted true protein content its proportion ammonia-N proportion, almost linearly dose effect. Sequencing analysis revealed 0.05) Sobs, Shannon, Chao Ace community relative abundance Enterobacter, Clostridium, Kosakonia decreased, Lactobacillus, Enterococcus Pantoea increased. Furthermore, Kosakonia, Terriglobus, Sphingomonas Sphingopyxis had an important role interaction network. is suggested application level 1–2% could improve quality via modifying